Data from: Synaptotagmin-1 serves as a primary Zn 2+ sensor to mediate spontaneous neurotransmitter release under pathological conditions

This dataset provides the source data for the paper titled "Synaptotagmin-1 serves as a primary Zn²⁺ sensor to mediate spontaneous neurotransmitter release under pathological conditions." Data includes electrophysiological patch-clamp recordings of miniature excitatory postsynaptic currents (mEPSCs), spontaneous EPSCs (sEPSCs), and evoked EPSCs, along with single-vesicle and ensemble lipid-mixing assays (in vitro reconstitution system) measuring fusion probability (%), cumulative probability, and lipid-mixing efficiency (FRET).

The dataset also covers zinc-protein or membrane-binding assays evaluating binding affinity and membrane-binding capacity, as well as vesicle docking imaging via TIRF microscopy, which quantifies docking-related fluorescence intensity and the number of docked vesicles. Data collection and analysis were conducted collaboratively across multiple institutions. Electrophysiological patch-clamp recordings and vesicle docking imaging were performed at the Key Laboratory of Xi'an Jiaotong University, while the remaining data were collected at the relevant experimental facilities of Sichuan University.