Full-length 16S rRNA gene long-read sequencing from dried cervicovaginal fluid

DNA was extracted from two 3 mm punches of paper cards containing dried cervicovaginal fluids that had been stored for up to eleven years at room temperature. Full-length 16S rRNA gene amplicons (~1.5 kb) were generated using the Kinnex 16S protocol followed by long-read sequencing using the Pacific Biosciences (PacBio) Revio system.

The raw fastq files consist of de-multiplexed and circular consensus sequencing reads. A more detailed description of the methods can be found in the paper.