Functional genomic study of the parotoid secretion from Bufo gargarizans

The fractionated peptides were subjected to biological screening. Also, the six tryptic peptides with large quantities derived from CL4590 were identified and they were named by their molecular weight, QUB-1132, QUB-2617, QUB-802, QUB-826, QUB-1153 and QUB-1260. Then, further chemically-synthesised were employed to evaluate their biological activities as well. <br>The results showed that RNA sequencing induced a low degree of sequence bias by a validation using specific primers.

It indicated that RNA-Seq is an authority technic for the acquisition of full-length sequence. On the other hand, none of these fractionated tryptic peptides had observable biological activities on three typical organisms, S. aureus, E.coil and C. albicans. In terms of synthesised peptides, QUB-826 is the only one who displayed antimicrobial activity against S. aureus and C. albicans.

However, QUB-1132 and QUB- 2617 exhibited anti-proliferative activity against several human cancer cell lines, especially on prostate cancer cell lines. These two tryptic peptides were supposed to be a factor of TRPM8, which might have relationship with cancer cells. The results indicated that the discovered tryptic peptides can be potential drug candidates.