Comparative transcriptomic analysis of GPC3 wild-type and knock-out HepG2 and Hep3B hepatocellular carcinoma cells 6 hours post-radiation

To elucidate the role of GPC3 in modulating radiation response, total RNA sequencing was performed on GPC3 wild-type (WT) and GPC3 knock-out (KO) HepG2 and Hep3B cell lines harvested 6 hours after radiation exposure. Comparative transcriptomic profiling across these 4 irradiated hepatocellular carcinoma cell line models identifies differential gene expression programs and potential signaling pathways regulated by GPC3 upon ionizing radiation.