Immune dysregulation in the prostates of C57BL/6Aire-/- mice mirrors that seen in BPH

The pathology is multifaceted, characterized by both stromal and epithelial cell proliferation, tissue fibrosis, lower urinary tract symptoms, and concurrent inflammatory processes. To model human benign prostatic hyperplasia (BPH), we employed 10X Genomics single-cell RNA sequencing to investigate prostate inflammation in C57BL/6 Aire-/- mice.The Aire-deficient mouse model provides a chronic inflammatory environment with intact immune responses, differing only in impaired central immune tolerance.

The resulting datasets were subsequently compared against existing human BPH single-cell RNA sequencing data to validate the model's relevance.