Dynamic alterations of bacterial and fungal microbiome following SRV-8 infection incynomolgus monkey

However, the dynamic alterations of gut microbiome following virus infection was scarcely reported. Here, we identified the SRV-8-infected monkeys and classified them into five groups according to the progression of SRV-8 infection. 16S rRNA amplicon sequencing demonstrated that the relative and inferred absolute abundance of bacterial genera UCG-002, Agathobacter, Coprococcus, and Holdemanella underwent significant changes at the early stage of SRV-8 infection (SRV-8 provirus formation stage), resulting in altered bacterial functions at the same stage.

ITS amplicon sequencing revealed that the fungal microbes showed no significant difference between the wild-type healthy and SRV-8-infected monkeys. Spearman correlation analyses indicated that the intestinal bacteria and fungi interacted closely following SRV-8 infection. Overall, our study contributes to a more comprehensive understanding about the dynamic alterations of bacterial and fungal microbes following SRV-8 infection and paves the way for deciphering the pathobiological mechanisms of SRV-8 infection from the perspective of gut microbiome.