Omics · study · 2026
Single-cell RNA sequencing of alveolar macrophages in GRP75 knockdown mice with SARS-CoV-2 N protein-induced lung injury
Listed in NCBI GEO
This study investigates the role of GRP75 (Hspa9)-mediated mitochondria-associated membranes (MAMs) in SARS-CoV-2 nucleocapsid (N) protein-induced acute lung injury.
Description
Using single-cell RNA sequencing of lung macrophages from control and GRP75 knockdown mice, we demonstrate that N protein promotes GRP75-dependent ER-mitochondria tethering, leading to mitochondrial calcium overload and proinflammatory macrophage activation.
GRP75 knockdown attenuates N protein-induced lung injury by reprogramming alveolar macrophage identity from a pro-inflammatory Car4+ state to a metabolically quiescent Hspa9-low state.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318978 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318978 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1420915 ncbi.nlm.nih.gov/bioproject/PRJNA1420915 ↗
project · from NCBI GEO
- PubMed 42570816 pubmed.ncbi.nlm.nih.gov/42570816 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE318978 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |