Omics · study · 2026
Circulating tRNA-Derived Fragments in Women with Endometriosis: A Pilot Study
Listed in NCBI GEO
Objective: To investigate whether circulating transfer RNA–derived fragments (tRFs) are differentially expressed in women with surgically confirmed endometriosis compared with healthy controls, and to assess the robustness and translational relevance of identified tRF signatures.
Description
Design: This exploratory case-control study analyzed circulating small RNA profiles in serum samples obtained from women with and without endometriosis.
Following RNA extraction and small RNA sequencing, tRF abundance was quantified and assessed for differential expression using established statistical frameworks. Robustness of findings was evaluated through complementary analytical methods and internal validation, and cross-species relevance was examined by comparison with previously published primate endometriosis data. Results: A total of 82 circulating tRFs were significantly differentially expressed between endometriosis and control groups (adjusted p < 0.05), including 19 upregulated and 63 downregulated fragments.
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The top 10 candidate tRFs exhibited large effect sizes (absolute log2 fold change range: −3.81 to +4.49). Effect size estimates were highly concordant across statistical methods (DESeq2 vs edgeR; Pearson r ≈ 0.99). Leave-one-out analysis demonstrated stability of effect direction across samples.
Seven tRFs with significant differential expression in humans demonstrated fully concordant regulation in a non-human primate endometriosis model, supporting biological relevance. Conclusion: Circulating tRF profiles differ between women with endometriosis and healthy controls and demonstrate robust statistical support and cross-species concordance. These results suggest that systemic tRF dysregulation could be a source of minimally invasive biomarkers.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343024 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343024 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1509620 ncbi.nlm.nih.gov/bioproject/PRJNA1509620 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE343024 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |