Data from: Self-assembled nanoclay gel with spheroid MSC-derived exosome mimetics to integrate demineralized bone matrix and noggin-targeting miRNA for synergistic osteogenesis

Here, we developed a multifunctional carrier system aimed at improving the capacity of DBM by utilizing nanoclay-based self-assembly along with cell-derived exosome mimetics (EMs). EMs were derived from mesenchymal stem cells (MSCs) osteogenically induced in spheroids and were functionalized with bisphosphonates (BP) to assemble a stable gel network with laponite nanoclays via BP-nanoclay edge interactions.

The resulting self-assembled nanoclay gel exhibited excellent injectability, moldability, and self-healing properties, even when loaded with a high concentration of DBM, and supported MSC osteogenic differentiation. We further enhanced the potency of DBM-mediated osteogenesis and bone morphogenetic protein (BMP) signaling in the nanoclay gel by incorporating BP-functionalized EMs loaded with miR-200c that can target BMP antagonist noggin.