Omics · study · 2026
Effect of disA overexpression on BCG transcriptome
Listed in NCBI GEO
This study investigates the transcriptomic consequences of graded intracellular cyclic di-AMP (c-di-AMP) production in Mycobacterium bovis BCG Danish 1331.
Description
Wild-type BCG Danish 1331 (BCG-WT) was used as the parental reference strain. Two recombinant strains overexpressing the Mycobacterium tuberculosis diadenylate cyclase gene disA (Rv3586) were generated.
In rBCG-V1, disA is expressed from the episomal plasmid pSD5-hsp60 under the control of the constitutive hsp60 promoter. rBCG-V2 carries an extrachromosomal recombinant plasmid, pKSB-hsp60-disA, containing two tandem copies of the hsp60-disA expression cassette, together with duplicated kanR and oriM sequences, while the chromosomal cdnP locus remains intact. These engineered strains generate progressively elevated intracellular c-di-AMP levels, with rBCG-V2 exhibiting higher c-di-AMP production than rBCG-V1.
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RNA sequencing was performed to characterize the global transcriptional changes associated with increasing c-di-AMP levels by comparing rBCG-V1 and rBCG-V2 with the parental BCG-WT strain.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE347nnn/GSE347518 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE347518 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1530427 ncbi.nlm.nih.gov/bioproject/PRJNA1530427 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE347518 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |