RNA-seq analysis of TGF-beta1-stimulated human RPTEC/TERT1 cells treated with mesenchymal stem cell-conditioned media

Human RPTEC/TERT1 cells were assigned to four conditions: untreated cells without TGF-beta1 or conditioned medium (NC); cells exposed to 10 ng/mL recombinant human TGF-beta1 for 24 h, followed by complete medium replacement with conditioned medium from immortalized human adipose-derived mesenchymal stem cells transfected with a negative-control miRNA mimic (TN) or a miR-29a-3p mimic (T29).

TGF-beta1 was maintained at 10 ng/mL throughout the subsequent 48 h conditioned-medium treatment. Bulk mRNA sequencing was used to characterize condition-associated transcriptomic changes.