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Data · dataset · 2019

Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota

Listed in Teesside University Research Data Repository

Raw 16S rRNA sequencing data from luminal contents from sections of the small (SI) and large intestines (LI) of 10 Swiss-Webster female mice (S+) that were infected with 200 S. mansoni cercariae and 10 age- and gender-matched mice that remained uninfected and were included as controls (S-).

Description

For each S+ and S- group, five mice were euthanised 28 days after infection (D28 p.i., i.e. before egg laying by sexually mature S. mansoni females had commenced).

The remaining mice (n = 5 for each S+ and S-) were euthanised at day 50 p.i. (D50 p.i., corresponding with ongoing egg laying by sexually mature S. mansoni females). The experiment was carried out in duplicate and samples from both experiments (i.e. Exp. 1 & Exp. 2) pooled for further analysis.

Read the rest (2 more)

The data set includes four types of files: 1. Raw joined read files - 1 per sample (demultiplexed): e.g. Exp1_SI_S-_D50_1.fasta 2. Joined, quality filtered file containing reads from all samples and ready for OTU picking.

The file was generated using Qiime: seqs.fna 3. Metadata file containing the relevant contextual information for each sample: Schisto_microbiome_Metadata.csv 4. OTU table created using Qiime including all of the taxonomic information: otu_table_mc2_w_tax_no_pynast_failures.biom

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Where it is published

Catalogue records · 1

Topics

Inferred from text
Evolutionary biology 72% · Sequencing 75% · Tabular 65%
Provenance · 1 source records, 14 field assertions
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Teesside University Research Data Repositoryoai:data.mendeley.com/y8c7vpc8zp.212 d agoJSON v1
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