Excel · study · 2026
STC-1 Attenuates Scar Formation via PI3K/AKT Pathway Activation and Immune Modulation
Listed in NCBI GEO
Objective: To investigate the role of Stanniocalcin-1 (STC-1) in scar formation and elucidate its underlying mechanisms in regulating fibroblast activity, immune microenvironment, oxidative stress, and PI3K/AKT signaling.
Description
Approach: A combination of clinical sample analysis, in vitro experiments using NIH-3T3 fibroblasts and macrophage co-culture models, RNA sequencing, and in vivo full-thickness wound mouse models were employed.
The effects of recombinant human STC-1 and STC-1 overexpression were evaluated on fibroblast function, extracellular matrix remodeling, inflammatory response, and scar formation. The impact on PI3K/AKT signaling was assessed by Western blot analysis. Results: STC-1 was specifically overexpressed in keloid tissues, primarily in fibroblasts, and its expression was upregulated by hypoxia in a HIF-1α–dependent manner.
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In vitro, STC-1 suppressed fibroblast proliferation, migration, and LPS-induced inflammation, while alleviating oxidative stress and mitochondrial dysfunction. STC-1 also promoted angiogenesis and induced M2 macrophage polarization. In vivo, STC-1 reduced scar size, improved collagen organization, modulated immune cell infiltration by activating the PI3K/AKT signaling pathway.
Innovation: This study identifies STC-1 as a novel immunoregulatory and anti-fibrotic factor in scar pathogenesis. It highlights that STC-1 not only affects fibroblast function but also remodels the immune microenvironment through PI3K/AKT activation. Conclusion: STC-1 exerts anti-fibrotic, anti-inflammatory, antioxidant, and immunomodulatory effects during scar formation by activating the PI3K/AKT signaling pathway, highlighting its therapeutic potential in the treatment of pathological scars.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303181 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303181 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1293396 ncbi.nlm.nih.gov/bioproject/PRJNA1293396 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE303181 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |