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Data · dataset · 2025

Data for: Resveratrol Targeting Smad4 as a Double-edged Sword Regulate Stem Cells-Mediated Regeneration

Listed in Teesside University Research Data Repository

RSV (CAS Number 501-36-0) was purchased from Aladdin and dissolved in DMSO (CAS Number 67-68-5), which was purchased from Thermo Scientific to obtain a stock solution.

Description

The stock solution was freshly diluted with artificial water to a final concentration of 1% DMSO. Based on preliminary experiments, we selected 5 μM and 50 μM as the concentrations for RSV exposure, with a control group (DMSO concentration <1%, which does not harm the worms) run in parallel.

In this experiment, planarians were placed in RSV-containing solutions at 20°C in the dark for 72 hours, with the solution renewed daily. At the end of the experiment, morphology and blastema size were recorded. Three replicates were set up for each group.

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Total RNA was extracted and sent to the BGI Genomics Institute for RNA sequencing (Shenzhen, China). The data were analyzed by the National Center for Biotechnology Information (ncbi.nlm.nih.gov/).

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Inferred from text
RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
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Teesside University Research Data Repositoryoai:data.mendeley.com/c8x65r4n35.112 d agoJSON v1
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