Omics · study · 2026
NUCB2/Nesfatin-1 promotes keratinocyte re-epithelialization by activating the mTORC1–SREBP2–HMGCR cholesterol biosynthesis axis
Listed in NCBI GEO
Cutaneous re-epithelialization is a rate-limiting step of wound healing and depends on the proliferation and migration of keratinocytes.
Description
NUCB2/Nesfatin-1 is a multifunctional peptide widely expressed in mammalian tissues, but its regulatory role in keratinocyte biology remains unclear. To investigate transcriptomic programs controlled by NUCB2, we performed bulk RNA sequencing of HaCaT keratinocytes after NUCB2 knockdown.
NUCB2 silencing significantly altered global gene expression patterns, most notably down-regulating sterol and cholesterol biosynthesis pathways including the SREBP2–HMGCR axis. This dataset provides a transcriptomic resource for exploring molecular mechanisms underlying NUCB2/Nesfatin-1-mediated epithelial regeneration.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311195 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311195 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1367328 ncbi.nlm.nih.gov/bioproject/PRJNA1367328 ↗
project · from NCBI GEO
- PubMed 42684001 pubmed.ncbi.nlm.nih.gov/42684001 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE311195 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |