Effect of shrimp hepatic enterocytes on intestinal and hepatopancreas flora of the Litopenaeus vannamei

PCR amplification was performed using a 10 ng DNA sample as a template. The reaction system was pre denatured at 98 ℃ for 1 minute, denatured at 98 ℃ for 10 seconds, annealed at 50 ℃ for 30 seconds, and extended at 72 ℃ for 30 seconds. 30 cycles were conducted, and finally extended at 72 ℃ for 5 minutes and stored at 4 ℃. Use the qiquick PCR purification kit (Qiagen, GmbH, Hilden, Germany) to merge and purify the PCR products of each sample, and use 1% agarose for gel electrophoresis verification of the PCR products.

Five sets of samples were sent for testing, including healthy shrimp gut (S.D.C), healthy shrimp hepatopancreas (S.D.G), diseased shrimp gut (S.X.C), diseased shrimp hepatopancreas (S.X.G), and water microbiota (S.S). Finally, sequencing was performed on the Illumina NovaSeq platform.