Data · dataset · 2026
Processed data and analysis code for: A Lactylation-Driven Post-Translational Enzyme Code Defines Microglial Functional Heterogeneity After Cerebral Ischemia-Reperfusion Injury
Listed in ScienceDB
This dataset contains the processed data and original analysis code associated with the research paper "A Lactylation-Driven Post-Translational Enzyme Code Defines Microglial Functional Heterogeneity After Cerebral Ischemia-Reperfusion Injury".
Description
The raw sequencing data is available at GEO under accession number GSEXXXXX.
Data description
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The dataset includes: 1) Seurat objects (.rds) containing processed single-cell transcriptomics data from GSE174574 and GSE227651, with PTM module scores and H3K9la target module scores. 2) BigWig files (.bw) for H3K9la ChIP-seq tracks for IGV visualization. 3) Peak files (.bed) from MACS2, including OGD-specific, Normal-specific, and common peaks. 4) A list of 5,175 OGD-specific H3K9la target genes (OGD_unique_target_genes.txt).
Methods
ChIP-seq reads were trimmed with Cutadapt v4.4 and aligned to the mm10 genome using Bowtie2 v2.5.5. PCR duplicates were removed with SAMtools v1.23.1. Peaks were called with MACS2 v2.2.9.1.
Single-cell data were processed with Seurat v5.0.3. The code to reproduce all analyses is provided in the "03_Code" directory.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.34072 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Biochemistry and cell biology 69% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.34072 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (69%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |