Omics · study · 2026
Decoding Immune Cell Subset Crosstalk in Biomaterial-Mediated Bone Regeneration: A Sequential Two-Stage Neutrophil-Macrophage Circuit
Listed in NCBI GEO
This study uses single-cell RNA sequencing to profile the early immune cell landscape during biomaterial-mediated bone regeneration in a rat calvarial defect model treated with a Janus microsphere delivery system.
Description
We identify a sequential two-stage immune circuit wherein CXCL2+ macrophages recruit pro-inflammatory N1 neutrophils via CXCL2-CXCR2 to initiate inflammation, while Neu2 neutrophils subsequently drive macrophage M2 polarization through Lgals9-CD44 to resolve inflammation and initiate osteogenesis-angiogenesis coupling.
These findings reveal novel immune subset crosstalk mechanisms and provide a theoretical basis for designing immunomodulatory bone repair materials.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344787 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344787 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1503606 ncbi.nlm.nih.gov/bioproject/PRJNA1503606 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE344787 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |