Omics · study · 2026
RNA-Seq of paired diagnosis and relapse AML specimens with healthy controls
Listed in NCBI GEO
Despite therapeutic advances, acute myeloid leukemia (AML) patients remains a clinically challenging malignancy, highlighting the continued need for identification of previously unrecognized targetable drivers of the disease to further improve current treatment options.
Description
Aberrantly expressed genes are associated with AML pathogenesis and can function as disease drivers. Targeting of their function or related pathways has shown promising results in identifying novel therapeutic targets.
We performed RNA sequencing (RNA-Seq) of AML patients specimens to identify aberrantly expressed AML genes associated with AML progression. The dataset comprised of two cohorts (I: n = 29 and II: n = 30), each cohort containing matched diagnosis-relapse pairs along with corresponding healthy control specimens (cohort I: n = 3 and cohort II: n = 5) derived from CD34+ normal bone marrow cells. All the diagnosis patients received combination intensive chemotherapy (7+3: continuous infusion of cytarabine for 7 days combined with anthracycline on days 1-3) during their induction treatment and all patients achieved remission after induction treatment.
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This sumission provides raw gene-level read counts to facilitate differential gene expression analyses between diagnosis and relapse specimens and relative to healthy controls. The fastq files for the data can be accessed through dbGap accession number phs001027.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327386 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327386 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1450626 ncbi.nlm.nih.gov/bioproject/PRJNA1450626 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE327386 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |