Data · dataset · 2025
Supplementary Fig. 5c & 6b | Western blots of FUS and TARDBP in iPSC lysates
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Project
Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution (su.figshare.com/projects/Early_mitochondrial_dysfunction_revealed_across_FUS-_and_TARDBP-ALS_at_single_cell_resolution/163252) From Data Availability Statement for the forthcoming paper in Nature Communications entitled: Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS "We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482.
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The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study. [Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files." -------------------------------------------- [Item specific description:] This data belong to Supplementary Fig. 5 and 6.
Western blots in iPSC lines with various FUS and TARDBP M337V mutations for FUS and TDP-43 protein expression. The assembled western blots are presented in the PPTX file. The fluorescent signal were scanned on a Odyssey CLx scanner (LI-COR) and exported as Image Studio ZIP archives.
More information about the Image Studio software can be obtained here: licorbio.com/image-studio The raw images are given as TIF files, thumbnails as PNG, and aquisition meta information as TXT files within the exported archives.
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Where it is published
- DOI doi.org/10.17045/sthlmuni.22555525 ↗
DOI / persistent id · from researchdata se
Catalogue records · 1
- OAI-PMH record api.researchdata.se/oai-pmh?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3A… ↗
metadata API · from researchdata se
Topics
- From keywords
- Earth & Environmental Science
- Inferred from text
- Image 75% · Imaging 75% · Microscopy 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 12 field assertions
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| concepts[modality].local:modality:rna-seq | enrichment · researchdata se | keyword-concept-rules@1.0.0 | title+description (75%) |
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| title | source · researchdata se | connector:researchdata_se@1.0.0 | /metadata/dc/title |