RNA-seq analysis of Hsf23a-overexpressing and Hsf23b-overexpressing maize plants for article "Alternative splicing of ZmHsf23 modulates maize heat tolerance by regulating sHSPs and TIL1 expression"

The WT, OE23L and OE23S maize seedlings that without or with 45 °C HS treatment for 1 h were used for RNA-seq. Three biological replicates of 6 samples were used to transcriptome profiling.Total RNA was extracted from the leaves using Total RNA Extraction Reagent (Vazyme). Transcriptome sequencing and data analysis were performed by Suzhou GENEWIZ Biotechnology Ltd (Suzhou, Jiangsu, China). The RNA-seq datasets also have been deposited in the National Center for Biotechnology Information (ncbi.nlm.nih.gov/) under accession number PRJNA1101208.