Supplementary Information accompanying proteomics manuscript

Figure S3. Heat maps of differentially expressed proteins annotated with protein names. Protein expression values have been log-transformed.

Figure S4. Representation of key metabolic pathways that are significantly affected by ocean acidification (A), mechanical stress at low pCO2 (B), and mechanical stress at high pCO2 (C). This includes input files and instructions for viewing data in iPath2.

Table S1. Raw and normalized (proportion) fatty acid data for 8 oysters each from 3 treatments: 400, 800, and 2800 µatm. Table S2.

ProteinProphet output for each technical replicate. Table S3. Protein expression values (NSAF) for each oyster for the 1 616 proteins identified.

Table S4. C. gigas proteins with associated SwissProt/UniProt-KB, Gene Ontology (GO), and GO Slim annotations. Table S5.

Enriched biological processes for proteins >2-fold differentially expressed in the stress responses to elevated pCO2 of 2800 µatm (“OA”), mechanical stress after a one month exposure to 400 µatm (“Mech Stress 400 µatm”), and mechanical stress after a one month exposure to 2800 µtam (“Mech Stress 2800 µatm”).