Effect of triazine thiols on steady-state mRNA levels in iPSC-derived hepatocytes

To determine whether these compounds affected hepatocyte mRNA levels, we performed bulk RNA sequencing of HLCs treated with the triazine thiol DL-1 or with vehicle (DMSO) for 24 hours. Treatment with triazine thiols only modestly affected steady-state mRNA levels.

Of 335 differentially expressed genes, none were directly related to ApoB synthesis, lipoprotein assembly, or secretory pathways. The the most affected mRNAs encoded proteins involved in metallothionein induction, suggesting a metal stress response likely unrelated to the compound’s effects on lipid metabolism. These results imply that DL-1 reduces APO-B secretion through post-transcriptional mechanisms.