Regulation of Immature Porcine Sertoli cell proliferation by tRNAGly-GCC and tRNAGlu-CTC-derived small RNAs

The abundance of tsRNA in porcine Sertoli cells accounted for 4.37% of the total small RNA, which was higher than that observed in spermatogonia and pachytene spermatocytes. Fourteen tsRNA families were found to be highly enriched in the mature Sertoli cells of porcine testis. Three tsRNAs, specifically Gly GCC 2-1, Glu CTC 1-1, and Glu CTC 1-6, were highly enriched in both mature and immature Sertoli cells.

Furthermore, YTHDF1 was confirmed as the target gene of Gly GCC 2-1, while CDK1 was identified as the target gene of Glu CTC 1-1. Both Gly GCC 2-1 and Glu CTC 1-1 were shown to reduce the viability of Sertoli cells and inhibit their proliferation. The findings of this study provide a foundational understanding for the development of porcine Sertoli cells and offer a theoretical reference for the regulation of spermatogenesis.