Nascent RNA profiling of MEF2B-mutant mantle cell lymphoma (MCL) to define immediate transcriptional programs

MEF2B, a transcription factor recurrently mutated in MCL, is implicated in oncogenic gene regulation, yet its immediate transcriptional effects are not well defined. Here, we use nascent RNA sequencing (PRO-seq) to characterize direct transcriptional consequences of MEF2B perturbation in MCL.

CRISPR-mediated depletion or mutagenesis of MEF2B is performed in MCL cell models to capture primary transcriptional changes before secondary effects occur. Nascent RNA mapping will identify MEF2B-dependent primary transcripts, which will be integrated with chromatin accessibility and histone modification datasets to define the regulatory networks controlled by MEF2B.