Effects of OGT-ISS deletion on gene expression in NK92 cells

In this study, we report that increasing O-GlcNAcylation enhances the tumor-killing efficacy of NK cells. By removing the ISS (intronic splicing silencer) of OGT in NK92 cells, we achieved stabilized O-GlcNAcylation even under tumor microenvironment (TME)-mimicking conditions, which led to a heightened killing effect against tumors.

To investigate how OGT-ISS deletion impacts gene expression profiles associated with NK cell activity, we performed RNA sequencing (RNA-seq) to compare the global transcriptomes of wild-type (WT) and ΔOGT-ISS_NK92 cells. This comparative transcriptome analysis aims to identify gene expression profiles that influence NK cell cytotoxicity and functional responses in the context of tumor immunity.