Single-cell transcriptome profiling of tracheal epithelium in basal cell-specific Mettl3 conditional knockout mice

To investigate the role of the RNA methyltransferase Mettl3 in airway basal cell homeostasis, we generated a comprehensive single-cell RNA sequencing atlas of tracheal epithelial cells from Mettl3 conditional knockout (M3-CKO) mice and littermate controls. Our dataset specifically focuses on Krt5-lineage traced basal cells, comparing tdTomato+ (traced) and tdTomato- (non-traced) populations from both genotypes.We present a comprehensive single-cell RNA-sequencing atlas of tracheas, comprising diverse populations of cells, epithelial cell, myeloid cells, B lymphocytes, T lymphocytes, endothelial cells, fibroblasts and glandular mucous cells.