Data · dataset · 2019
R1 Supplemental dataset.xlsx
Listed in DataCite
Whole transcriptome analysis of TGF-beta1 treated fibroblasts
Description
Methods
RNA was purified using a miRCURY RNA isolation kit (#300110, Exiqon A/S, Vedbaek, Denmark) with an Agilent 2100 Bioanalyzer (Agilant, Santa Clara, CA, USA) used to check RNA integrity. The Illumina TrueSeq mRNA sample preparation kit (Illumina, San Diego, CA, USA) was used to prepare RNA libraries for sequencing on the Illumina HiSEq 2000. Reads were aligned using TopHat2 with the rn6 build of the rat genome.
Read the rest (1 more)
Transcript assembly and differential expression (DE) was by Cufflinks with Refseq mRNAs to guide assembly. Statistically different changes were identified by ANOVA using Partek Flow (Partek, Singapore), as previously described in doi.org/10.1016/j.biocel.2017.09.009.
Links
Where it is published
- Repository landing page figshare.com/articles/R1_Supplemental_dataset_xlsx/7464818 ↗
landing page · from DataCite
- Repository landing page figshare.com/articles/R1_Supplemental_dataset_xlsx/7464818/1 ↗
landing page · from DataCite
- DOI doi.org/10.6084/m9.figshare.7464818 ↗
DOI / persistent id · from DataCite
- DOI doi.org/10.6084/m9.figshare.7464818.v1 ↗
DOI / persistent id · from DataCite
Documentation and papers
Catalogue records · 4
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.7464818.v1 ↗
metadata API · from DataCite
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.7464818 ↗
metadata API · from DataCite
- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.7464818.v1 ↗
catalogue entry · from DataCite
- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.7464818 ↗
catalogue entry · from DataCite
Topics
- Stated by source
- Basic medicine · Basic medicine · Clinical medicine · Clinical medicine
- Inferred from text
- Sequencing 75%
Provenance · 2 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.7464818 | 15 d ago | JSON v1 |
| DataCite | 10.6084/m9.figshare.7464818.v1 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| byte_size | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:basic-medicine | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:basic-medicine | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:clinical-medicine | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:clinical-medicine | source · DataCite | connector:datacite@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · DataCite | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · DataCite | connector:datacite@1.0.0 | |
| description | source · DataCite | connector:datacite@1.0.0 | /data/attributes/descriptions |
| license | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| publication_date | source · DataCite | connector:datacite@1.0.0 | /data/attributes/dates |
| title | source · DataCite | connector:datacite@1.0.0 | /data/attributes/titles/0/title |
| updated_date | source · DataCite | connector:datacite@1.0.0 |