Transcriptomic changes associated with GPNMB knockdown in HGC-27 gastric cancer cells

Cells were transiently transfected with either GPNMB-targeting siRNA or a non-targeting control siRNA. RNA sequencing was performed using three independently prepared biological samples per group.

Differential-expression and enrichment analyses were used to identify candidate biological processes and signaling pathways associated with GPNMB knockdown. Several pathways, including cAMP signaling, appeared among the nominal enrichment outputs. The cAMP pathway was therefore treated as a hypothesis-generating candidate for subsequent experimental investigation.