Amplicon sequencing of soil fungi detected within the mixed coniferous forest of the western Sierra Nevada, California, USA; inferred from ITS1 amplicon sequencing with ITS1f-ITS2 primers

We used high-throughput DNA sequencing of the Internal Transcribed Spacer region (ITS1) to quantify the richness and abundance of fungi in 144 soil samples collected from 12 forest stands at the Blodgett Forest Research Station in the western Sierra Nevada, California.

Beginning in 2001, each forest stand was randomly assigned to one of four forestry treatments: prescribed fire only, mechanical thinning only, mechanical plus fire, and control. Fire only treatments were applied in 2002, 2009, and 2016. Mechanical only treatments occurred in 2002 and 2017.