Genome-wide Identification of Host miRNAs Interacting with RIG-I via RIP-seq Using RIG-I as Bait

At 24 h post-transfection, cells were harvested and lysed, followed by RNA immunoprecipitation (RIP) assay using an anti-Flag antibody. After the specific pull-down of Flag-tagged RIG-I protein complexes by the anti-Flag antibody was verified via Western blotting (WB), total RNA was extracted from the RIP-enriched complexes and subjected to next-generation sequencing (NGS), to identify the types and relative abundance of host miRNAs bound to Flag-RIG-I.