Omics · study · 2026
TNF-blockade attenuates the proinflammatory CRS signature of monocyte-derived macrophages.
Listed in NCBI GEO
TNF-blockade abates cytokine release, weight loss and eventual lethality in our preclinical model of CAR T cell-induced Cytokine Release Syndrome (CRS).
Description
Macrophages are known mediators in the pathophysiology of CRS. We generated this single-cell RNA sequencing data set to assess the effect of TNF on monocytes and macrophages in CRS.
The data show that upon CRS-induction there is an increase in monocyte-derived (Ccr2-positive) macrophages at the tumor site. In an unperturbed CRS these monocyte-derived macrophages show high levels of Ly6C and accordingly have a proinflammatory phenotype expressing known mediators of CRS-pathophysiology Nos2 and Il1b. Under TNF-blockade the emerging monocyte-derived macrophages show low levels of Ly6C and lack this proinflammatory phenotype.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335046 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335046 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1477156 ncbi.nlm.nih.gov/bioproject/PRJNA1477156 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE335046 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |