Large-scale megabarcoding using Oxford Nanopore Technology enables fine-scale mapping of <i>Terebellides</i> (Annelida, Trichobranchidae) in Sweden with the description of two new species

The first one used individual Sanger sequencing to obtain a 313 bp fragment of the COI marker. The second method combined tagged primers and multiplexed sequencing with Oxford Nanopore Technology (ONT). A total of 191 sequences were obtained with Sanger sequencing and 413 sequences with ONT, demonstrating the advantage of the latter method for this type of dataset.

Six species were found, including <i>Terebellides europaea, T. lavesquei, T. shetlandica</i> and <i>T. williamsae</i> and new specimens of two species that had previously been detected but remained undescribed. They are here described as <i>Terebellides agreniusi</i> sp. nov. and <i>Terebellides nevmyanovi</i> sp. nov.</p> <p>LSID for the publication: <a target="_blank" href="urn:lsid:zoobank.org:pub:9D13BD18-9D25-4327-8139-F46273449BD9">urn:lsid:zoobank.org:pub:9D13BD18-9D25-4327-8139-F46273449BD9</a></p> <p>LSID for <i>T. agreniusi:</i> <a target="_blank" href="urn:lsid:zoobank.org:act:012F26B8-D297-4F43-9F45-33230BE1B158">urn:lsid:zoobank.org:act:012F26B8-D297-4F43-9F45-33230BE1B158</a></p> <p>LSID for <i>T. nevmyanovi:</i> <a target="_blank" href="urn:lsid:zoobank.org:act:261E60E9-265F-457A-BE43-ADFF995A9C3A">urn:lsid:zoobank.org:act:261E60E9-265F-457A-BE43-ADFF995A9C3A</a></p> <p></p><p>Oxford Nanopore Technology is an efficient and cost-effective method for large-scale barcoding and mapping of benthic invertebrates.</p><p>Two new species of <i>Terebellides</i> are described from Russia and Sweden.</p><p>A synoptic table for species of <i>Terebellides</i> present in the Skagerrak and Kattegat is given.</p><p></p> <p>Oxford Nanopore Technology is an efficient and cost-effective method for large-scale barcoding and mapping of benthic invertebrates.</p> <p>Two new species of <i>Terebellides</i> are described from Russia and Sweden.</p> <p>A synoptic table for species of <i>Terebellides</i> present in the Skagerrak and Kattegat is given.</p>