Data · dataset · 2026
Discovery and functional evaluation of a host defense peptide from Rana amurensis
Listed in ZivaHub and Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.17034/32629992.v1
Due to the drug-resistant bacterial infections have become a global medical problem, antimicrobial peptides (AMPs) are considered as a new possibility to replace traditional antibiotics.
Description
Host-defense peptides in amphibians skins with various biological activities are a rich source for discovering new AMPs. This thesis amid to discover and functionally study a new AMP from the skin secretions of Rana amurensis.
A cDNA encoding a new ranatuerin precursor was successfully established through molecular cloning and Sanger sequencing, and the mature peptide sequence of the active form, QUB-2566 (GVLKGVGKNVSGSLLHQLKCLISGGC), was deduced from the cloned cDNA. Thereafter, the corresponding synthetic peptide was obtained by solid-phase Fmoc synthesis, and its antibacterial activity, antiproliferative activity and haemolytic activity were tested.
Read the rest (2 more)
QUB-2566 was found to inhibit the growth of S. aureus and E. coli from a peptide concentration of 1µM; however, it required higher peptide concentration to completely inhibit the growth of these bacteria. Interesting, this peptide exhibited better bactericidal effect against E.coli (MBC=32 μM) than grampositive bacterium (S.aureus) (MBC=128 μM). Moreover, this peptide had less potency against C.albicans (MIC=512μM), along with low haemolytic activity (HC50< 512 μM).
Also, peptide QUB-2566 had a weak antiproliferative effect on lung cancer cells H838. Altogether, this peptide may become a lead compound for new antimicrobial drug development because of its potent antibacterial potency against E.coli and low haemolytic activity.
Links
Where it is published
- DOI doi.org/10.17034/32629992.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75% · Sequencing 75%
Provenance · 4 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32629992 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32629992 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32629992 | 13 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/32629992 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |