Omics · study · 2026
sRNA sequencing of Lactococcus cremoris in response to penicillin and lysozyme stress
Listed in NCBI GEO
The contribution of small RNAs to the regulation of cell wall defense mechanisms, which play a central role in protecting bacteria from environmental insults, remains poorly understood in Lactococcus cremoris, a paradigmatic organism for studying lactic acid bacteria.
Description
Analysis of the profiles of 193 putative sRNAs, including 131 newly identified in this study, revealed altered expression of 20 and 25 sRNAs following treatment with major cell wall-targeting antimicrobials lysozyme and penicillin G, respectively.
Further analysis and genome-wide functional screening identified four sRNAs that modulate the lysozyme response in opposite directions, with sLLM1042+, sLLM2-, and sLLM1993+ conferring increased, and sLLM461+ decreased, resistance. Moreover, elevated expression of sLLM2- or sLLM1042+ promoted the sensitivity of cells to penicillin G and certain abiotic stresses, and had pleiotropic effects on gene regulation at both RNA and protein levels, positioning them as key elements in regulatory networks.
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Notably, the sLLM2- mediated decrease in four putative tellurium resistance proteins resulted in L. cremoris sensitivity to tellurite stress. As sLLM2- mutations, altering lysozyme and penicillin responses, reduce the expression of alanine racemase Alr, we suggest that its upregulation possibly modulates D-alanine levels, thereby affecting lipoteichoic acid modification or peptidoglycan synthesis. Our findings underscored the broad roles of sRNAs in defensive responses to cell wall-targeting antimicrobials in lactic acid bacteria.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299210 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299210 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1273082 ncbi.nlm.nih.gov/bioproject/PRJNA1273082 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE299210 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |