Omics · study · 2026
Effect of Tyrphostin-AG4178 on ex-vivo mouse ovarian follicles in eIVFG ovulation system
Listed in NCBI GEO
Ovulation is a coordinated, multicellular process carried out in the antral follicle involving gonadotropin-stimulus, cumulos-oocyte complex expansion, follicle rupture, followed by luteinization.
Description
Ovulation can be prevented by targeted inhibition of upstream signalling molecules via tool compounds such as Tyrphostin-AG4178, which binds to EGFR and thus prevents downstream MEK/ERK, prostaglandin, and Pgr signaling. We exposed ex vivo mouse ovarian follicles grown in an encapsulated in-vitro follicle growth (eIVFG) system to Tyrphostin-AG4178 after stimulus with hCG and performed bulk-RNA sequencing on whole follicles to observe the transcriptomic response to targeted ovulation inhibition.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335098 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335098 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1477402 ncbi.nlm.nih.gov/bioproject/PRJNA1477402 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE335098 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |