Omics · study · 2026
Single-Cell Transcriptomic Profiling of Immune Cells from Parental and EMP1-Deficient MB49 Bladder Tumors.
Listed in NCBI GEO
In this study, we employed a single-cell RNA sequencing (scRNA-seq) approach using the 10x Genomics platform to investigate the immune microenvironment of syngeneic mouse bladder tumors.
Description
Tumors were established in immunocompetent C57BL/6 mice using parental or EMP1-knockout MB49 bladder cancer cells. Tumor-infiltrating immune cells were isolated and profiled at single-cell resolution to define immune cell populations, transcriptional states, and functional programs within the tumor microenvironment.
Comparative analyses were performed to determine how tumor-cell EMP1 expression influences immune cell composition, activation and exhaustion states, inflammatory signaling, and intercellular communication. This dataset provides a single-cell transcriptomic resource for investigating EMP1-mediated regulation of antitumor immunity in bladder cancer.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343966 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343966 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1514019 ncbi.nlm.nih.gov/bioproject/PRJNA1514019 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE343966 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |