Original data of intestinal flora

It aims to investigate the effects of dietary emulsifiers—sodium carboxymethyl cellulose (CMC)—in combination with the organophosphate pesticide chlorpyrifos (CPF) on gut microbiota composition and function. The study further validates the mechanistic role of gut microbes via fecal microbiota transplantation (FMT) and sodium butyrate supplementation.

All experiments were conducted in SPF-grade animal facilities at China Agricultural University. Detailed experimental methods are described in the associated manuscript.This dataset includes raw gut microbiota data from the following three components:Exposure experiment (The effects of food emulsifiers on chlorpyrifos-induced intestinal inflammation): Mice were administered different combinations of CPF and emulsifiers (CMC or soy lecithin, SL) across six treatment groups.Fecal microbiota transplantation (FMT): Fecal samples from exposed mice were transferred into microbiota-depleted mice (via antibiotic pretreatment) to assess functional consequences of altered microbial communities.Sodium butyrate supplementation: Butyrate was administered under CPF exposure conditions to evaluate its effect on restoring microbial and immune homeostasis.Microbial DNA was extracted from ileal and colonic contents.