Bulk RNA sequencing of tyrosine hydroxylase-knockdown human EGFRvIII-directed CAR T cells in vitro and after in vivo recovery

For the in vitro experiment, donor-matched 2173-BBζ-CON-KD and 2173-BBζ-TH-KD CAR T-cell products were independently generated from three healthy human donors, stimulated with EGFRvIII extracellular domain-coated beads, and collected on day 3 for bulk RNA sequencing.

For the in vivo experiment, the two CAR T-cell products were generated from the same human donor and administered to separate U87-EGFRvIII-bearing NSG-SGM3 recipient mice. GFP-positive human CAR T cells were sorted separately from the spleen of each mouse 3 days after CAR T-cell infusion, with three independent recipient mice per condition and no pooling. The submission includes paired-end raw sequencing data and gene-level raw-count matrices for both experiments.