Omics · study · 2026
Preliminary Study on the Role of lncRNA ENST00000608721 in Mycobacterium tuberculosis Infection of Macrophages
Listed in NCBI GEO
To identify long non-coding RNAs (lncRNAs) associated with Mycobacterium tuberculosis (M.tb) infection, and preliminarily investigate the role of lncRNA ENST00000608721 in macrophages during M.tb infection.
Description
Methods
Total RNA was extracted from M.tb-infected and uninfected THP-1 cells, and high-throughput sequencing was performed on the Illumina platform to obtain the transcriptional profile. The differential lncRNAs were subjected to Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, respectively. Furthermore, the differential lncRNAs were verified by real-time quantitative PCR (qPCR).
Read the rest (1 more)
A THP-1 cell model with silenced target lncRNA was constructed using siRNA chemical transfection, and the bacterial load after M.tb infection was determined by colony-forming units (CFU) assay.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322582 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322582 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1430583 ncbi.nlm.nih.gov/bioproject/PRJNA1430583 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE322582 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |