Fig. 2b-c | Quantitative immunofluorescence microscopy for intracellular FUS localization in ALS motor neurons

The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study. [Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files." ------------------------- [Item specific description:] Quantititative fluorescene microscopy for FUS protein in ALS motor neurons The dataset are Harmony archives for the high content imager Opera Phenix (Perkim Elmer).

The proprietary harmony software creates a database with file organization stored in xml and oar files and folders with raw tif files. More information here on our imaging platform: kclwcic.co.uk/operaphenix These archives contain thousands of tif files. Therefore, they are compressed into tar.gz files with matching md5 checksums for verification.

The prism file is just a graphpad prism file with the summary data. More info here: graphpad.com/features