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Data · dataset · 2015

Effects of ABA and CaCl 2 on GABA accumulation in fava bean germinating under hypoxia-NaCl stress

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Effects of exogenous abscisic acid (ABA) and CaCl 2 on γ-aminobutyric acid (GABA) accumulation of germinated fava bean under hypoxia-NaCl stress were investigated.

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Exogenous ABA resulted in the enhancement of glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA content in cotyledon and shoot. CaCl 2 increased both enzyme activities in shoot and GABA content in cotyledon and shoot.

ABA downregulated GAD expression in cotyledon and radicle, while upregulated that in shoot; it also upregulated DAO expression in each organ. CaCl 2 upregulated GAD expression in cotyledon, while downregulated that in radicle. However, it upregulated DAO expression in shoot, downregulated that in radicle.

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ABA inhibitor fluridon and ethylenediaminetetraacetic acid inhibited GAD and DAO activities significantly so that inhibited GABA accumulation through reducing ABA biosynthesis and chelating Ca 2+ , respectively. However, they upregulated GAD and DAO expression in varying degrees. These results indicate that ABA and Ca 2+ participate in GABA biosynthesis in fava bean during germination under hypoxia-NaCl stress.

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Chemistry · Life Sciences
Provenance · 1 source records, 12 field assertions
SourceKeyLast seenRaw
DataCite10.6084/m9.figshare.162123515 d agoJSON v1
FieldAssertionExtractorEvidence
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concepts[field].fos:biological-sciencessource · DataCiteconnector:datacite@1.0.0
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concepts[field].local:field:chemistrymapping · DataCitevocabulary-mapper@1.0.0keywords['Chemistry']
concepts[field].local:field:life-sciencesmapping · DataCitevocabulary-mapper@1.0.0keywords['Biological Sciences']
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