Excel · study · 2026
Transcriptome sequencing analysis of Cyp4f3b gene interference in human gallbladder carcinoma cells gbc-sd
Listed in NCBI GEO
This study aimed to investigate the molecular mechanism underlying the effects of Cyp4f3b gene interference on the biological behaviors of human gallbladder carcinoma cells gbc-sd.
Description
Three replicate cell samples from each group (sh-NC control group and sh-Cyp4f3b interference group) were subjected to total RNA extraction and quality control, sequencing library construction and evaluation, RNA-seq sequencing and filtering, reference sequence alignment, gene expression level analysis, and differential expression analysis.
Differentially expressed genes and significantly enriched signaling pathways were systematically identified to provide molecular evidence for clarifying the role of Cyp4f3b in gallbladder carcinogenesis and progression.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325472 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325472 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1440241 ncbi.nlm.nih.gov/bioproject/PRJNA1440241 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE325472 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |