Omics · study · 2026
Bulk RNA-seq of WNT-responsive and WNT-secreting cancer cell populations from orthotopic PDAC tumors
Listed in NCBI GEO
Pancreatic ductal adenocarcinoma (PDAC) exhibits pronounced intratumoral heterogeneity driven in part by WNT signaling dynamics.
Description
To characterize transcriptional differences among distinct WNT signaling states in vivo, we performed bulk RNA sequencing of fluorescence-activated cell sorting (FACS)-isolated cancer cell populations from orthotopic PDAC tumors. WNT-responsive (WNT-R), WNT-secreting (WNT-S), double-positive (WNT-R/S), and double-negative (DN) cancer cells were isolated from KPC 7TCF-eGFP/Porcn-DMA reporter tumors.
RNA libraries were prepared using the SMARTerSeq2 protocol and sequenced from four biological replicates per group. Differential gene expression analysis revealed enrichment of canonical WNT/β-catenin target genes in WNT-R cells, elevated Porcn expression in WNT-S cells, and intermediate transcriptional profiles in WNT-R/S cells, validating the fidelity of the reporter system in vivo. This dataset provides a resource for dissecting WNT-dependent cancer cell states and signaling interactions in PDAC.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316194 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316194 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1401785 ncbi.nlm.nih.gov/bioproject/PRJNA1401785 ↗
project · from NCBI GEO
- PubMed 41875882 pubmed.ncbi.nlm.nih.gov/41875882 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE316194 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |