Omics · study · 2026
Matrix-Bound Nanovesicles in Alcohol-Related Liver Disease: Characterization and Therapeutic Application
Listed in NCBI GEO
Background & Aims: Alcohol-related liver disease (ALD) lacks effective therapies, and understanding extracellular matrix (ECM) remodeling during disease progression may reveal novel therapeutic targets.
Description
Matrix-bound nanovesicles (MBVs), embedded within ECM, contain bioactive proteins and microRNAs and promote tissue repair in various injury models. However, MBVs remain unexplored in ALD.
In the present study, we not only characterized how alcohol alters hepatic MBV composition, but also evaluated therapeutic effects of exogenous healthy MBVs in alcohol-induced liver injury. Approach: Using the NIAAA chronic-plus-binge ethanol model, we first isolated hepatic MBVs and performed LC-MS/MS proteomic analysis with pathway enrichment to define alcohol-induced cargo alterations. We then administered MBVs from healthy porcine urinary bladder matrix to ethanol-exposed mice and assessed therapeutic effects through histopathological evaluation and bulk RNA sequencing.
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Results and Conclusions: Ethanol exposure enriched hepatic MBVs for metabolic stress and detoxification proteins, indicating disease-associated cargo remodeling. Therapeutic administration of healthy MBVs reduced steatosis, lipid peroxidation, and fibrogenic gene expression while normalizing ethanol-induced transcriptional programs related to oxidative stress, inflammation, and metabolism. MBVs acted context-dependently.
That is, they promoted mild ECM remodeling in healthy livers while attenuating pathological signaling in injured tissue, suggesting they stabilize hepatocyte homeostasis rather than directly suppressing immunity. These findings establish MBVs as both disease-responsive biomarkers and therapeutic modulators of the hepatic microenvironment in ALD.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316773 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316773 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1404176 ncbi.nlm.nih.gov/bioproject/PRJNA1404176 ↗
project · from NCBI GEO
- PubMed 42520960 pubmed.ncbi.nlm.nih.gov/42520960 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · Mass spectrometry 65% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE316773 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:mass-spectrometry | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |