Data · dataset · 2015
An Efficient Regeneration System and Optimization of the Transformation from the Cotyledonary Node of Jute ( Corchorus capsularis L.)
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The purpose of this study was to use cotyledonary nodes as explants to establish an efficient regeneration protocol for jute ( Corchorus capsularis L.) on Murashige and Skoog (MS) basal medium.
Description
Research into the different growth regulators using the orthogonal design L16 (4 5 ) revealed that the best shoot induction medium is MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6BA and 0.25 mg/L NAA.
The average number of shoots per explant and the explant induction rate were 9.8 and 100%. After 3 weeks, 2–3 cm shoots were rooting on 1/2 MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6-BA + 0.2 mg/L NAA. Moreover, we optimized Agrobacterium -mediated transformation using the GUS gene transient expression system.
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The best condition for obtaining higher transformation rate consisted of the use of fresh explants to which 100 μM acetosyringone was added for a co-culture time of 10 min, the OD value of Agrobacterium liquid is 0.5 at 600 nm. These data provide an important basis for the application of other trait gene in the improvement of jute fiber quality.
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Where it is published
- Repository landing page tandf.figshare.com/articles/dataset/An_Efficient_Regeneration_System_and_Optimiza… ↗
landing page · from DataCite
- DOI doi.org/10.6084/m9.figshare.1454499 ↗
DOI / persistent id · from DataCite
Documentation and papers
- Creative Commons Attribution 4.0 International creativecommons.org/licenses/by/4.0/legalcode ↗
license · from DataCite
- IsSupplementTo 10.1080/15440478.2014.912972 doi.org/10.1080/15440478.2014.912972 ↗
publication · from DataCite
Catalogue records · 2
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.1454499 ↗
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- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.1454499 ↗
catalogue entry · from DataCite
Topics
- Stated by source
- Biological sciences · Health sciences
- From keywords
- Chemistry · Life Sciences
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.1454499 | 15 d ago | JSON v1 |
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|---|---|---|---|
| access_level | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| byte_size | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:biological-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:health-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · DataCite | vocabulary-mapper@1.0.0 | keywords['Chemistry'] |
| concepts[field].local:field:life-sciences | mapping · DataCite | vocabulary-mapper@1.0.0 | keywords['Biological Sciences'] |
| created_date | source · DataCite | connector:datacite@1.0.0 | |
| description | source · DataCite | connector:datacite@1.0.0 | /data/attributes/descriptions |
| license | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| publication_date | source · DataCite | connector:datacite@1.0.0 | /data/attributes/dates |
| title | source · DataCite | connector:datacite@1.0.0 | /data/attributes/titles/0/title |
| updated_date | source · DataCite | connector:datacite@1.0.0 |