Omics · study · 2026
PGK1 moonlights as an NF-kB/p65 kinase to orchestrate M2-like macrophage-mediated immunosuppression in triple-negative breast cancer
Listed in NCBI GEO
Triple-negative breast cancer (TNBC) is the most aggressive molecular subtype with limited therapeutic options.
Description
Metabolic reprogramming is a hallmark of cancer, and emerging evidence indicates metabolic enzymes possess non-canonical moonlighting functions. We identified phosphoglycerate kinase 1 (PGK1) as a key link between glycolysis and immunosuppression in TNBC.
To investigate the role of PGK1 in shaping the tumor immune microenvironment, we performed single-cell RNA sequencing (scRNA-seq) on TNBC cells (4T1) with PGK1 knockdown (shPgk1) and negative control (shNC), each with three biological replicates. Single-cell libraries were prepared on the TaiM4 scRNA platform and sequenced on the BGI DNBSEQ platform using paired-end sequencing. A total of 33,138 high-quality cells were retained after quality filtering.
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Our analysis revealed that PGK1 knockdown significantly altered the transcriptional landscape and immune signaling pathways in TNBC cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341921 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341921 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1504824 ncbi.nlm.nih.gov/bioproject/PRJNA1504824 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE341921 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |