Omics · study · 2026
RNA-seq profiling of LPS tolerance in monocytes, THP-1, fibroblasts, smooth muscle cells, and endothelial cells
Listed in NCBI GEO
Atherosclerosis – a chronic disease of the arteries characterized by lipid accumulation and chronic local inflammation – is the primary cause of most ischemic cardiovascular diseases.
Description
The inflammatory process can be limited by endotoxin tolerance. This study performed a comparative transcriptomic analysis of LPS tolerance in primary monocytes, the THP-1 monocyte line, fibroblasts, endothelial cells, and smooth muscle cells.
To model LPS tolerance, single and double LPS stimulation was performed. Sequencing of libraries obtained from total RNA after poly(A) selection was performed on the Illumina NextSeq 2000 platform. Primary monocytes and smooth muscle cells developed LPS tolerance; fibroblasts showed partial, mediator-dependent tolerance; THP-1 cells showed limited tolerance primarily for TNF; endothelial cells did not show tolerance for IL-8/CCL2.
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No conserved transcriptional tolerance signature was observed across tolerant cell types; overlap was limited to three genes (OAS1, IFIT2, IFIT3).
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327840 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327840 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1452864 ncbi.nlm.nih.gov/bioproject/PRJNA1452864 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE327840 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |