Data · dataset · 2024
Whole genome data of PDX1 mutant cynomolgus macaques and its parents
Listed in ScienceDB
Genomic DNA was extracted from the pancreas of the M4 fetus and the blood of its father and mother using a TIANamp Genomic DNA Kit (Tiangen, DP304).
Description
The genomic DNA (200 ng) was subjected to whole-genome sequencing at a sequencing depth of 30–40 × using the Illumina HiSeq 2000 platform at the Novogene Bioinformatics Institute (Beijing, China). Potential off-target sites (POTs) for each sgRNA were predicted to analyze site-specific edits according to Cas-OFFinder.
Initially, all potential off-target sites in the mutant monkeys were compared with the reference genome. The filtered potential off-target sites were then compared to the parent genomes. After filtering, the final potential off-target sites were obtained and confirmed by Sanger sequencing
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.11703 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Genetics 70% · Genome sequencing 65% · Sequencing 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.11703 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3105 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (70%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:genome-sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |