Text · study · 2026
Mitochondrial Ca2+ homeostasis controls inter-compartmental proteostasis in Arabidopsis plants [Ribo-seq]
Listed in NCBI GEO
Purpose: The goals of this study are to determine the effects of mitochondrial Ca2+ homeostasis on cytosolic translation
Description
Methods
The monosomal and polysomal mRNA profiles of 10-day-old WT, MCU2 overexpression and mcu sextuple mutation plants were generated by deep sequencing, in triplicate, using Illumina HiSeq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks Results: Using an optimized data analysis workflow, we mapped a minimum of 23 million sequence reads per sample to the Arabidopsis genome (TAIR10) and identified more than 23,000 transcripts.
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Data analysis revealed that perturbation of mitochondrial Ca2+ altered specifically the translational efficiency of RNA modification genes Conclusions: Our study represents the first detailed analysis of Arabidopsis monosomal and polysomal RNA transcriptomes in response to impaired mitochondrial Ca2+, with biologic replicates, generated by RNA-seq technology. Our results showed that perturbation of mitochondrial Ca2+ triggered cytosolic translation response.
We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses of inter-compartmental communication
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE186nnn/GSE186417 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE186417 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA773765 ncbi.nlm.nih.gov/bioproject/PRJNA773765 ↗
project · from NCBI GEO
- PubMed 42207405 pubmed.ncbi.nlm.nih.gov/42207405 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Arabidopsis thaliana · Other
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE186417 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:3702 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |